Preparation and in vitro and in vivo characterization of composite microcapsules for cell encapsulation.
نویسندگان
چکیده
Cell encapsulation technology raises great hopes in medicine and biotechnology. Transplantation of encapsulated pancreatic islets represents a promising approach to the final cure of type 1 diabetes mellitus. Unfortunately, long-term graft survival and functional competence remain only partially fulfilled. Failure was often ascribed to the lack of biocompatibility generating inflammatory response, limited immunobarrier competence, hypoxia, and low beta-cell replication. In the present work, ketoprofen loaded biodegradable microspheres, embedded into alginate/poly-L-ornithine/alginate microcapsules, were prepared in order to release ketoprofen at early stages after implantation. Morphology, size, in vitro release behaviour, and in vivo biocompatibility were assessed. The effect of some preparation parameters was also evaluated. Polymeric microspheres were spherical and smooth, two populations of about 5 and 20 microm of mean diameter characterized the particle size distribution. A high burst effect was observed for all preparations during in vitro release studies. Ketoprofen, plasticizing the polymeric matrix, could be responsible of this release behaviour. Alginate/poly-L-ornithine/alginate microcapsules were not modified upon ketoprofen loaded microspheres encapsulation and an optimal dispersion was obtained. Composite system showed good biocompatibility when a high molecular weight polymer was employed. Therefore a potentially suitable composite system for cell encapsulation was obtained. This system may be successfully used to release NSAIDs and other active molecules capable to improve cell system functional performance and life-span.
منابع مشابه
Development and Characterization of Bioadhesive Gel of Microencapsulated Metronidazole for Vaginal Use
The present study concerned with the development and characterization of metronidazole microcapsules prepared by thermal change method using different ratios (1:1, 1:2 and 1:4) of ethyl cellulose in order to select the best microcapsule formulation with a good encapsulation efficiency and drug release profile. The obtained microcapsules were discrete, spherical with free flowing properties and ...
متن کاملDevelopment and Characterization of Bioadhesive Gel of Microencapsulated Metronidazole for Vaginal Use
The present study concerned with the development and characterization of metronidazole microcapsules prepared by thermal change method using different ratios (1:1, 1:2 and 1:4) of ethyl cellulose in order to select the best microcapsule formulation with a good encapsulation efficiency and drug release profile. The obtained microcapsules were discrete, spherical with free flowing properties and ...
متن کاملPLGA Nanospheres Loaded with Autoclaved Leishmania Major (ALM) and CpG-ODN: Preparation and in vitro Characterization
Objective(s) Several antigens, adjuvants and delivery systems have been evaluated for induction of protective immune responses against Leishmaniasis, but most of them have been inefficient. In this study, PLGA nanospheres as antigen delivery system CpG-ODN as an immunoadjuvant for increasing the immune responses against Autoclaved Leishmania major (ALM) were prepared and characterized. Materi...
متن کاملPreparation, in-vitro characterization and pharmacokinetic evaluation of Brij decorated doxorubicin liposomes as a potential nanocarrier for cancer therapy
The aim of current study was to investigate the effect of Brij decoration of liposomes on in vitro and in vivo characteristics of the nanocarriers. Two hydrophilic Brij surfactants (Brij 35 and Brij 78) with almost similar molecular weight but differing in acyl chain were incorporated into liposomal bilayers at two percentages (5% and 10%). Conventional liposomes (CL) containing egg phosphatidy...
متن کاملPreparation, in-vitro characterization and pharmacokinetic evaluation of Brij decorated doxorubicin liposomes as a potential nanocarrier for cancer therapy
The aim of current study was to investigate the effect of Brij decoration of liposomes on in vitro and in vivo characteristics of the nanocarriers. Two hydrophilic Brij surfactants (Brij 35 and Brij 78) with almost similar molecular weight but differing in acyl chain were incorporated into liposomal bilayers at two percentages (5% and 10%). Conventional liposomes (CL) containing egg phosphatidy...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- International journal of pharmaceutics
دوره 324 1 شماره
صفحات -
تاریخ انتشار 2006